caski cc cell lines  (ATCC)

Journal: Bioengineered

Article Title: Transcriptional factor 3 binds to sirtuin 1 to activate the Wnt/β-catenin signaling in cervical cancer

doi: 10.1080/21655979.2022.2076481

Figure Lengend Snippet: TCF3 is possibly linked to unfavorable prognosis of CC. A, expression of TCF3 in pan cancers; B, expression of TCF3 in GSE7803 and GSE9750 datasets; C, distribution of TCF3 in CC tissues and normal cervical tissues in The Human Protein Atlas system; D, TCF3 expression in TCGA-CESC; E, association of TCF3 expression with the patient survival in TCGA-CESC; F, TCF3 mRNA expression in CC cell lines (CaSki, C-33A, SiHa, SW756 and HeLa) and in normal End1/E6E7 cells detected by RT-qPCR (* p < 0.05; ** p < 0.01; one-way ANOVA). Data are expressed as the mean ± SD.

Article Snippet: Human CC cell lines CaSki, SiHa, C-33A, SW756, and HeLa, and a human immortalized cervical epithelial cell-line End1/E6E7 were procured from American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: Bioengineered

Article Title: Transcriptional factor 3 binds to sirtuin 1 to activate the Wnt/β-catenin signaling in cervical cancer

doi: 10.1080/21655979.2022.2076481

Figure Lengend Snippet: Interactions between TCF3/SIRT1/β-catenin in CC cells. A, SIRT1 expression and β-catenin nuclear accumulation in CC cell lines (CaSki, C-33A, SiHa, SW756 and HeLa) and normal End1/E6E7 cells examined by western blot analysis (* p < 0.05, two-way ANOVA); B, SIRT1 expression and β-catenin nuclear accumulation in HeLa and SiHa cells transfected with si-TCF3 determined by western blot assay (* p < 0.05, two-way ANOVA); C, expression of SIRT1 and nuclear accumulation of β-catenin in HeLa and SiHa cells after SIRT1-OE transfection and XAV-939 treatment examined by western blot assay (*# p < 0.05, two-way ANOVA, * compared to si-TCF3 + SIRT1-NC; # compared to SIRT1-OE + DMSO); D, cell proliferation determined by colony formation assay (*# p < 0.05, two-way ANOVA, * compared to si-TCF3 + SIRT1-NC; # compared to SIRT1-OE + DMSO); E, migration of cells determined by Transwell assay (*# p < 0.05, two-way ANOVA, * compared to si-TCF3 + SIRT1-NC; # compared to SIRT1-OE + DMSO); F, apoptosis rate in HeLa and SiHa cells detected by flow cytometry (*# p < 0.05, two-way ANOVA, * compared to si-TCF3 + SIRT1-NC; # compared to SIRT1-OE + DMSO). Data are expressed as the mean ± SD.

Article Snippet: Human CC cell lines CaSki, SiHa, C-33A, SW756, and HeLa, and a human immortalized cervical epithelial cell-line End1/E6E7 were procured from American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Western Blot, Transfection, Colony Assay, Migration, Transwell Assay, Flow Cytometry

Journal: Journal of Genomics

Article Title: Circ-0036602 Acts As a Sponge of MiR-34a-5p and MiR-431-5p to Promote Cervical Cancer Proliferation and Invasion

doi: 10.7150/jgen.62458

Figure Lengend Snippet: Circ0036602 was upregulated in CC. (A) A total of 516 differentially expressed cirRNAs in Caski-shRNA-E7, Siha-shRNA-E7, and negative control were identified via cirRNAs RNA sequencing. (B-C) Kyoto Encyclopedia of Gene and Genome pathway and Gene Ontology analysis of differentially expressed genes in cervical cancer. (D) The relative expression of circ0036602 in HPV16 positive and HPV negative CC tissue samples. (E-G) The correlated analysis between the relative expression of circ0036602 and differentiation, FIGO stage, and N stage. (H) Kaplan-Meier curves of overall survival of 30 CC patients with low or high circ0036602 expression. (I) The relative expression of circ0036602 in CC cells performed by qRT-PCR. *p< 0.05, Values represent mean± SD, n= 3 independent experiments.

Article Snippet: CC cell lines Caski, Siha, C33A and normal cervical epithelial Hacat, purchased from American Type Culture Collection (ATCC), were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin at 37°C with 5% carbon dioxide.

Techniques: shRNA, Negative Control, RNA Sequencing, Expressing, Quantitative RT-PCR

Journal: Journal of Genomics

Article Title: Circ-0036602 Acts As a Sponge of MiR-34a-5p and MiR-431-5p to Promote Cervical Cancer Proliferation and Invasion

doi: 10.7150/jgen.62458

Figure Lengend Snippet: The characterization of circ0036602 in CC. (A) The schematic illustration showed that exons 5~10 of ZNF592 constitute circ0036602. (B) The presence and circular form of circ0036602 established by agarose gel electrophoresis. GAPDH acts as the linear control. (C) circ0036602 and ZNF592 mRNA expression in Caski and Siha cells treated with or without R detected by qRT-PCR analysis. (D-E) The nuclear and cytoplasmic distribution of circ0036602 assessed by qRT-PCR and FISH assay in. *p< 0.05, Values represent mean± SD, n= 3 independent experiments.

Article Snippet: CC cell lines Caski, Siha, C33A and normal cervical epithelial Hacat, purchased from American Type Culture Collection (ATCC), were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin at 37°C with 5% carbon dioxide.

Techniques: Agarose Gel Electrophoresis, Control, Expressing, Quantitative RT-PCR

Journal: Journal of Genomics

Article Title: Circ-0036602 Acts As a Sponge of MiR-34a-5p and MiR-431-5p to Promote Cervical Cancer Proliferation and Invasion

doi: 10.7150/jgen.62458

Figure Lengend Snippet: HMGB1 was a target of miR-34a-5p and miR-431-5p. (A) Venn diagram demonstrated the 10 genes from miRbase, TargetScan, Miranda, and RNA sequencing. (B-C) The relative expression of 10 genes in Caski and Siha cells detected by qRT-PCR. (D-E) Western blot verified the expression of HMGB1 in Caski and Siha cells. (F) Correlation analysis between circ0036602 and HMGB1. (G-H) The negative intersection with miR-34a-5p and miR-431-5p and HMGB1. *p< 0.05, Values represent mean± SD, n= 3 independent experiments.

Article Snippet: CC cell lines Caski, Siha, C33A and normal cervical epithelial Hacat, purchased from American Type Culture Collection (ATCC), were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin at 37°C with 5% carbon dioxide.

Techniques: RNA Sequencing, Expressing, Quantitative RT-PCR, Western Blot

Journal: Molecular Medicine Reports

Article Title: hsa_circ_0101119 facilitates the progression of cervical cancer via an interaction with EIF4A3 to inhibit TCEAL6 expression

doi: 10.3892/mmr.2021.12293

Figure Lengend Snippet: hsa_circ_0101119 is highly expressed in CC tissues and cells. (A) Heatmap of differentially expressed circRNAs in CC tissues and normal tissues according to the online data set (GSE102686). (B) Expression level of hsa_circ_0101119 was detected via reverse transcription-quantitative PCR in CC cell lines (C-33A, SiHa, CaSki and HeLa) and normal human cervical epithelial cell line, HcerEpic. **P<0.01 vs. HcerEpic cells. (C) Expression level of hsa_circ_0101119 in CC tissues and normal tissues, according to the online data set (GSE102686). circRNA/circ, circular RNA.

Article Snippet: The four human CC cell lines (C-33A, SiHa, CaSki and HeLa) and the normal human cervical epithelial cell line, HcerEpic, were supplied by VCANBIO Cell & Gene Engineering Co., Ltd. All the cells were cultured in RPMI 1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) FBS (Invitrogen; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.), and cultured at 37°C in a humidified 5% CO 2 incubator.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: hsa_circ_0101119 facilitates the progression of cervical cancer via an interaction with EIF4A3 to inhibit TCEAL6 expression

doi: 10.3892/mmr.2021.12293

Figure Lengend Snippet: hsa_circ_0101119 recruits EIF4A3 to inhibit TCEAL6 expression in CC. (A) Bioinformatics was used to predict the interaction probabilities of the RNA-binding protein EIF4A3 with hsa_circ_0101119. Predictions with probabilities >0.5 were considered ‘positive’, suggesting that the corresponding RNA and protein are likely to interact. (B) RIP assay using anti-EIF4A3 showed that EIF4A3 precipitated hsa_circ_0101119 in SiHa and HeLa cell lysates. (C) Pull down assay indicated that biotin-labeled hsa_circ_0101119 interacted with EIF4A3. (D) Bioinformatics was used to predict the interaction probabilities of EIF4A3 with TCEAL6. (E) RIP assay using anti-EIF4A3 showed that EIF4A3 precipitated TCEAL6 in SiHa and HeLa cell lysates. (F) Expression levels of EIF4A3 and TCEAL6 were detected via RT-qPCR in CC cell lines (C-33A, SiHa, CaSki and HeLa) and a normal human cervical epithelial cell line, HcerEpic. (G) Expression levels of EIF4A3 and TCEAL6 in CC tissues and normal tissues, according to the analysis of TCGA. (H) Correlation between EIF4A3 and TCEAL6 in CC samples from TCGA. (I) After transfection with sh-EIF4A3, RT-qPCR was used to detect EIF4A3 expression in SiHa and HeLa cells. (J) After transfection with sh-EIF4A3, western blotting was performed to detect the expression level of TCEAL6 in SiHa and HeLa cells. (K) After co-transfection with si-hsa_circ_0101119 and sh-EIF4A3, western blotting was performed to measure the expression level of TCEAL6 in SiHa and HeLa cells. (L) A proposed model whereby hsa_circ_0101119 sequesters EIF4A3 away from TCEAL6 mRNA, in turn suppressing TCEAL6 mRNA translation. **P<0.01 vs. IgG group (B and E); *P<0.05, **P<0.01 vs. HcerEpic cells group (F); *P<0.05 vs. normal tissues group (G); **P<0.01, vs. sh-NC group (I and J); **P<0.01 vs. sh-NC group, ## P<0.01, vs. si-hsa_circ group. (K) RIP, RNA immunoprecipitation; RT-qPCR, reverse transcription-quantitative PCR; TCGA, The Cancer Genome Atlas; sh, short hairpin RNA; NC, negative control; si, small interfering RNA; circ, circular RNA; EIF4A3, eukaryotic initiation factor 4A-3; TCEAL6, transcription elongation factor A-like 6; T, tumor; N, normal; CC, cervical cancer.

Article Snippet: The four human CC cell lines (C-33A, SiHa, CaSki and HeLa) and the normal human cervical epithelial cell line, HcerEpic, were supplied by VCANBIO Cell & Gene Engineering Co., Ltd. All the cells were cultured in RPMI 1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) FBS (Invitrogen; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.), and cultured at 37°C in a humidified 5% CO 2 incubator.

Techniques: Expressing, RNA Binding Assay, Pull Down Assay, Labeling, Quantitative RT-PCR, Transfection, Western Blot, Cotransfection, Immunoprecipitation, Real-time Polymerase Chain Reaction, shRNA, Negative Control, Small Interfering RNA